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KMID : 0613820110210050671
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Journal of Life Science
2011 Volume.21 No. 5 p.671 ~ p.677
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Isolation and Characterization of a Calmodulin-binding Ca²?-ATPase 2 (SCA2) in Soybean
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Park Hyeong-Chul
Kim Ho-soo
Lee Sang-min
Cho Hyeon-Seol
Chung Woo-sik
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Abstract
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We previously reported the isolation and characterization of a gene, SCA1 (for soybean Ca²?-ATPase 1), encoding a calmodulin-regulated Ca²?-ATPase that is located in the plasma membrane in soybean. Here, a Ca²?-ATPase designated as SCA2 was isolated from soybean. The two Ca²?-ATPases, SCA1 and SCA2, share a remarkably high degree of similarity (78%). Ten transmemebrane domains were predicted by hydropathy analysis. Using gel overlay assays, CaM was found to bind to SCA2 in a Ca²?-dependent manner. Southern blot analysis revealed the presence of two copies of the Ca²?-ATPase gene in the soybean genome. An N-terminal truncation mutant that deletes sequence through the putative calmodulin binding site was able to complement a yeast mutant (K616) that was deficient in two endogenous Ca²? pumps. Our results indicate that SCA2 is structurally highly conserved with type IIB Ca²? pumps in plants.
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KEYWORD
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Calcium, Ca²?-ATPase, Calmodulin, plasma membrane, soybean (Glycine max)
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